Quantifying Expansion Microscopy with DNA Origami Expansion Nanorulers

Abstract

In the past decade super-resolution microscopy1,2 developed rapidly and allowed seeing new structural details in fluorescence microscopy, especially in the field of bioimaging3-6. Most of the evolving techniques like (d)STORM7,8, STED9 or SIM10 - just to name a few - focused on overcoming the diffraction limit11 by increasing the technical resolution of the microscope, often in combination with specifically designed probes, for example by using blinking fluorescent dyes7,8,12,13. But the approach to improve the effective microscope resolution of a system is not the only way to reveal new insights. An alternative approach is based on physically expanding the sample ...