bioRxivBioengineeringPreprint
Ex-STORM: Expansion Single Molecule Nanoscopy
Zhisong Tong, Paolo Beuzer, Qing Ye, Josh Axelrod, Zhenmin Hong, Hu Cang
Abstract
We present critical improvements to recently invented Expansion Microscopy (ExM), which resolve the incompatibility between ExM and single molecule super-resolution microscopy STORM. Specifically, the improved ExM circumvents the massive, 50-100%, bleaching of fluorophores during sample preparations, and preserve the efficiency of enzymatic oxygen scavenging systems in expanded samples. These improvements open up new avenues for Ex-STORM - expanding a sample mechanically, and then visualizing the sample with STORM.
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