RSC PharmaceuticsBioengineeringPeer-reviewed journal article
Sequential- vs. density gradient- centrifugation for the isolation of mitochondria-containing extracellular vesicles
Kandarp M. DaveiD, Bodhi T. Brady, Balaji GovindaswamyiD, Vivek BasudkariD, Donna Beer StolziD, Devika Soundara ManickamiD
Abstract
A subset of extracellular vehicles (EVs) with particle diameters >200 nm, large vesicles (lEVs) contain mitochondria that increase recipient cell bioenergetics. To date, sequential centrifugation (SC) is the most reported protocol to separate lEVs from the smaller EVs ( View larger version (38K): org.highwire.dtl.DTLVardef@102b68borg.highwire.dtl.DTLVardef@11e7df1org.highwire.dtl.DTLVardef@de7161org.highwire.dtl.DTLVardef@1e5d64c_HPS_FORMAT_FIGEXP M_FIG C_FIG
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