Multi-area single-cell calcium imaging dataset of the mouse cortex across wakefulness, sleep, and anesthesia

Abstract

We present a reusable dataset of neuronal population activity from multiple cortical areas, recorded from layers 2/3 of the mouse cortex at single-cell resolution during wakefulness, natural sleep (including NREM and REM sleep), and isoflurane anesthesia. Using wide-field two-photon microscopy, we recorded approximately 4,000 to 10,000 neurons per session at 7.65 Hz and provided the spatial coordinates of individual neurons. The repository provides both processed datasets and the corresponding raw imaging movies (TIFF) and electrophysiological recordings (MATLAB format). Processed data are distributed in MATLAB format and include {Delta} F/F fluorescence signals, deconvolved spike estimates, Gaussian-smoothed spike estimates, behavioral state annotations, and metadata. This dataset supports reuse in studies of cortical population dynamics, brain-state-dependent activity, and spatially distributed neuronal organization. It should also be useful for method development, benchmarking, and comparative analyses of large-scale neuronal activity across physiological and pharmacological brain states.