Liangyi Chen

This paper introduces SIM-fdFLIM, a super-resolution FLIM method that preserves ratio accuracy and enables high-speed, high-resolution live-cell lifetime imaging.

This study shows that repeated pup exposure converts virgin male mice to a parental state via calcitonin receptor-expressing neurons in the medial preoptic area.

The paper introduces COMET, a lightweight head-mounted mesoscope for cortex-wide single-cell calcium imaging in freely moving mice, demonstrating its ability to capture neural activity during natural behaviors.

The paper introduces MorphAgent, a biologically grounded AI framework for cell profiling across microscopy modalities, improving feature design and performance in drug screening and disease analysis.

This paper introduces CLG, a structural-functional calibration method for volumetric calcium imaging that corrects neuron identity errors, reducing duplicate counts and improving network analysis accuracy.

The paper shows that δ-cell interactions modulate pancreatic islet Ca2+ oscillation patterns through somatostatin secretion and pharmacological/optogenetic perturbations, revealing a Hopf bifurcation mechanism.

This paper introduces FLAME, a high-throughput 3D ultrasound imaging technique that significantly improves resolution and speed for microvascular imaging in mouse organs.

This study shows that glycemia shifts induce a coordinated change in pancreatic islet Ca2+ oscillations, mediated by δ- and α-cells, which is impaired in diabetes.

This paper develops a novel peripherally acting alpha2A-adrenergic receptor antagonist, CDS479-2, which improves glucose control in diabetic mice without central side effects.

This paper introduces SN2N, a self-supervised denoising method for live-cell super-resolution microscopy that achieves competitive performance without requiring large clean datasets.

The paper develops GRAB sensors for detecting neuropeptides with high specificity and sensitivity, enabling in vivo monitoring of their release and function.

The paper shows that pancreatic α and β cells exhibit globally phase-locked Ca2+ oscillations with tunable phase shifts that set islet oscillation patterns.

The paper examines the spatial organization of neuron types in the medial entorhinal cortex and adjacent parasubiculum using two-photon microscopy, finding that grid cells cluster separately from other spatial cell types.

This study uses light-sheet imaging to map the origin of vascular endothelial cells in zebrafish gastrulae, revealing contributions from different regions along developmental axes.

The paper develops an optimized GRABACh sensor to measure in vivo cholinergic activity in Drosophila and mice, revealing compartmental ACh signals and single-trial dynamics.

This study uses in vivo imaging in zebrafish to show that β-cell maturation occurs in two waves, initiated by low glucose and boosted by increased glucose delivery, with calcineurin/NFAT activation playing a key role.