bioRxivNeurosciencePreprint
Protocol for simultaneous evaluation of neuronal activity and neurotransmitter release following chronic amyloid-beta oligomer injections into the hippocampus
Vincent HervéiD, Laurie Bonenfant, Mathilde Amyot, Rime Balafrej, Obaï Bin Ka’b AliiD, Habib BenaliiD, Jonathan BrouilletteiD
Abstract
In Alzheimers disease, there is an imbalance in neurotransmitter release and altered neuronal activation. We present a novel approach to analyze neuronal activity by combining local field potential (LFP) recording with microdialysis within the same animal. This method measures glutamate and GABA levels following chronic hippocampal amyloid-beta oligomer (A{beta}o) injections in rats. We outline the design of our electrode and canula, the surgical procedure, and the steps for LFP recording, interstitial fluid collection, and A{beta}o injections simultaneously in living animal. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=182 HEIGHT=200 SRC="FIGDIR/small/614333v2_ufig1.gif" ALT="Figure 1"> View larger version (37K): org.highwire.dtl.DTLVardef@155f3c0org.highwire.dtl.DTLVardef@1f60289org.highwire.dtl.DTLVardef@207ba2org.highwire.dtl.DTLVardef@94eeb3_HPS_FORMAT_FIGEXP M_FIG C_FIG
- Citations
- 0
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Manipulation of neuronal activity in the entorhinal-hippocampal circuit affects intraneuronal amyloid-β levels
Christiana Bjorkli, Nora Cecilie Ebbesen, … Ioanna Sandvig·bioRxiv·Jul 5, 2022
- Identification of amyloid beta oligomers in locus coeruleus (LC) neurons of Alzheimer's patients and their impact on LC oxidative stress, inhibitory neurotransmitter receptors and neuronal excitability
Louise Kelly, Mohsen Seifi, … Jerome D. Swinny·Neuropathology and Applied NeurobiologyPeer-reviewed·Oct 29, 2020
- Riluzole shifts glial responses to protect synapses and memory in Aβ oligomer-treated rats
Min-Kaung Wint-Mon, Hiroyuki Kida, … Dai Mitsushima·bioRxiv·Jan 30, 2026
- Quantification of astrocytic synaptic pruning in hippocampus in response to in vitro Aβ oligomer incubation via colocalization analysis with C1q
Arpit Kumar Pradhan, Qinfang Shi, … Gerhard Rammes·bioRxiv·May 9, 2022
- Functional spreading of hyperexcitability induced by human and synthetic intracellular Aβ oligomers
Eduardo J. Fernández-Pérez, Braulio Muñoz, … Luis Gerardo Aguayo·bioRxiv·Oct 16, 2020
- Astrocyte-neuron interplay is critical for Alzheimer's disease pathogenesis and is rescued by TRPA1 channel blockade
Adrien Paumier, Sylvie Boisseau, … Mireille Albrieux·BrainPeer-reviewed·Jul 22, 2021