bioRxivPlant BiologyPreprint
Rapid expression of COVID-19 proteins by transient expression in tobacco.
P. L. Lindsay, A. Ackerman, Y. Jian, O. Artz, D. Rosado, T. Skopelitis, M. Kitagawa, U. V. Pedmale, D. Jackson
Abstract
In 2020 we suffered from a major global pandemic caused by the SARS-CoV-2 coronavirus. Efforts to contain the virus include the development of rapid tests and vaccines, which require a ready supply of viral proteins. Here we report the production of two SARS-CoV-2 proteins by transient transformation of tobacco, leading to high expression within three days, and subsequent purification of the intact proteins. Such efforts may help to develop testing resources to alleviate the major impacts of this global pandemic.
- organism: plant (other)
- assay: proteomics
- research focus: drug response / drug discovery
- approach: experimental assay
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