L. Martinez-Sobrido

SARS-CoV-2 nsp15 endoribonuclease antagonizes dsRNA-induced antiviral signaling

This study shows that SARS-CoV-2 nsp15 inhibits dsRNA-induced antiviral signaling, which is necessary for viral replication in nasal epithelial cultures.

Multiple layers of innate immune response antagonism of SARS-CoV-2

This study generates twelve SARS-CoV-2 viruses with mutations in individual proteins and shows that many have interferon-antagonizing activity and immunosuppressive effects in human cells and mice, with NSP1 and NSP15 having the greatest impact.

Viral Syncytia Evolve to Resist Interferon

This study shows that SARS-CoV-2 syncytia reduce interferon and antibody effectiveness, explaining the evolution of fusogenic viral variants.

Activation of Protein Kinase R (PKR) Plays a Pro-Viral Role in Mammarenavirus Infected Cells

This study shows that PKR activation can both restrict and enhance LCMV replication, revealing a complex role for PKR in mammarenavirus infection.

The SARS-CoV-2 Spike is a virulence determinant and plays a major role on the attenuated phenotype of Omicron virus in a feline model of infection

This study shows the Omicron BA.1 Spike protein reduces SARS-CoV-2 virulence in a feline model by impairing viral spread and causing less severe disease.

Antivirals against monkeypox infections

This study evaluates 19 compounds for antiviral activity against Orthopoxviruses, identifying 13 with activity against vaccinia virus and 11 with activity against monkeypox virus.

A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication and pathogenesis in vivo

This study shows that mutating the SARS-CoV-2 NSP3 Mac1 domain reduces viral replication and pathogenesis in vivo.

Transmissible SARS-CoV-2 variants with resistance to clinical protease inhibitors

The paper identifies SARS-CoV-2 variants resistant to protease inhibitors nirmatrelvir and ensitrelvir, showing pre-existing resistance and potential for spread.

Monitoring SARS-CoV-2 infection using a double reporter-expressing virus

This paper introduces a dual-reporter SARS-CoV-2 virus that expresses both mCherry and Nluc for improved in vitro and in vivo infection monitoring.

Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis.

This study shows SARS-CoV-2 ORF6 disrupts nucleo-cytoplasmic trafficking, inhibits IFN signaling, and modulates viral protein expression, with variant polymorphisms affecting its function.

Structure and Epitope of a Neutralizing Monoclonal Antibody that Targets the Stem Helix of β Coronaviruses

This paper determines the crystal structure of a broadly neutralizing antibody targeting the stem helix of coronaviruses and elucidates its mechanism of neutralization.

Using a reverse genetics system to generate recombinant SARS-CoV-2 expressing robust levels of reporter genes

This paper describes a BAC-based reverse genetics system to generate reporter-expressing recombinant SARS-CoV-2 for studying viral pathogenesis and drug screening.

Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for therapy

This study shows that Numb-associated kinases are essential for SARS-CoV-2 infection and that inhibiting them can suppress viral infection in human lung cells.

Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission

This study develops a live-attenuated SARS-CoV-2 vaccine by deleting two accessory proteins, showing it protects against viral challenge and transmission in animal models.

Potent universal-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibody

This study identifies a potent universal coronavirus neutralizing monoclonal antibody, 1249A8, effective against multiple variants and demonstrating therapeutic activity through intranasal administration.

Vaccinia virus attenuation by codon deoptimization of the A24R gene for vaccine development

This study attenuates vaccinia virus by codon deoptimization of the A24R gene, creating a safe and protective vaccine candidate against poxvirus infection.

A single amino acid substitution in PB1 of pandemic H1N1 with A/Ann Arbor/6/60 master donor virus mutations as a novel live-attenuated influenza virus vaccine

This paper shows that a single amino acid change in the PB1 protein of the pandemic H1N1 virus can create a live-attenuated vaccine with reduced pathogenicity and transmission.

Bi-reporter vaccinia virus for tracking viral infections in vitro and in vivo

This paper introduces bi-reporter vaccinia viruses expressing both fluorescent and luciferase genes to track viral infections in vitro and in vivo, enabling detailed study of infection dynamics and host-pathogen interactions.

SARS-CoV-2 hijacks p38β/MAPK11 to promote viral protein translation

This study identifies p38β as a critical host factor for SARS-CoV-2 replication and shows that inhibiting it reduces viral replication and cytokine production.

A SARS-CoV-2 nucleocapsid protein TR-FRET assay amenable to high-throughput screening

The paper develops a TR-FRET assay for SARS-CoV-2 NP detection, enabling high-throughput screening of compounds against SARS-CoV-2 and its variants.

A bifluorescent-based assay for the identification of neutralizing antibodies against SARS-CoV-2 variants of concern in vitro and in vivo

This paper develops a bifluorescent-based assay to identify neutralizing antibodies against SARS-CoV-2 variants in vitro and in vivo, showing it accurately reflects viral neutralization.

Visualization of SARS-CoV-2 infection dynamic

This study develops a method to visualize SARS-CoV-2 infection dynamics using reporter-expressing recombinant viruses, enabling real-time tracking and antibody screening in vivo.

4'-Fluorouridine is a broad-spectrum orally efficacious antiviral blocking respiratory syncytial virus and SARS-CoV-2 replication

This paper identifies 4'-Fluorouridine as an orally effective antiviral that inhibits RSV and SARS-CoV-2 replication in vivo.

Modeling SARS-CoV-2 and Influenza Infections and Antiviral Treatments in Human Lung Epithelial Tissue Equivalents

The paper validates in vitro human lung tissue models for studying SARS-CoV-2 and influenza virus infections and antiviral treatments.

SARS-CoV-2 B.1.1.7 and B.1.351 variants of concern induce lethal disease in K18-hACE2 transgenic mice despite convalescent plasma therapy

This study compares the pathogenicity and immune response of SARS-CoV-2 variants B.1.1.7 and B.1.351 with WA-1 in mice, showing reduced protection from convalescent plasma against the variants.

Amino acid residues involved in inhibition of host gene expression by influenza A/Brevig Mission/1/1918 PA-X

The paper identifies amino acid residues critical for PA-X's host gene expression inhibition using a bacteria-based system.

Rapid and Efficient Inactivation of SARS-CoV-2 from Surfaces using UVC Light Emitting Diode Device

This paper describes a UVC LED device that rapidly inactivates SARS-CoV-2 on surfaces, achieving log10 reductions in viral titers within seconds.

Epitope classification and RBD binding properties of neutralizing antibodies against SARS-CoV-2 variants of concern

This study classifies NAb epitopes against SARS-CoV-2 RBD and evaluates how variants affect NAb and ACE2 binding, identifying key mutations that impact neutralization and infectivity.

A metal ion orients mRNA to ensure accurate 2'-O ribosyl methylation of the first nucleotide of the SARS-CoV-2 genome

The paper reports structures of the SARS-CoV-2 nsp16/nsp10 enzyme complex before and after RNA product release, revealing conformational changes and the role of a metal ion in 2'-O methylation of the first nucleotide.

Contribution of SARS-CoV-2 accessory proteins to viral pathogenicity in K18 hACE2 transgenic mice

This study identifies ORF3a and ORF6 as key contributors to SARS-CoV-2 pathogenesis in mice using a reverse genetics system and K18 hACE2 transgenic mice.