Mutational dynamics and transmission properties of SARS-CoV-2 superspreading events in Austria
A. Popa, J.-W. Genger, M. Nicholson, T. Penz, D. Schmid, S. W. Aberle, B. Agerer, A. Lercher, L. Endler, H. Colaco, M. Smyth, M. Schuster, M. Grau, F. Martinez Jimenez, O. Pich, W. T. Borena, E. Pawelka, Z. Keszei, M. Senekowitsch, J. Laine, J. Aberle, M. Redlberger-Fritz, M. Karolyi, A. Zoufaly, S. Maritschnik, , A. Bergthaler
Abstract
Superspreading events shape the COVID-19 pandemic. Here we provide a national-scale analysis of SARS-CoV-2 outbreaks in Austria, a country that played a major role for virus transmission across Europe and beyond. Capitalizing on a national epidemiological surveillance system, we performed deep whole-genome sequencing of virus isolates from 576 samples to cover major Austrian SARS-CoV-2 clusters. Our data chart a map of early viral spreading in Europe, including the path from low-frequency mutations to fixation. Detailed epidemiological surveys enabled us to calculate the effective SARS-CoV-2 population bottlenecks during transmission and unveil time-resolved intra-patient viral quasispecies dynamics. This study demonstrates the power of integrating deep viral genome sequencing and epidemiological data to better understand how SARS-CoV-2 spreads through populations. Graphical Abstract O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY
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