RFC1 AAGGG pentanucleotide repeats form parallel G-quadruplex: structural implications for aberrant molecular cascades in CANVAS

Abstract

A pentanucleotide repeat expansion (PRE) of (AAGGG)n in the replication factor C subunit 1 (RFC1) gene is recently identified as the genetic cause of cerebellar ataxia, neuropathy, and vestibular areflexia syndrome (CANVAS), and also linked to several other neurodegenerative disorders including the Parkinsons disease. However, the molecular mechanism by which RFC1 PRE drives pathology remains poorly understood. Here, for the first time we discovered and determined the high-resolution structures of parallel G-quadruplex formed by AAGGG repeats within the pathogenic RFC1 PRE, revealing an intriguing conformational plasticity at the 3-termi that allows stacking of multiple G4s. We further identify a molecular mechanism by which the DNA G4 in RFC1 PRE impedes polymerase processivity leading to replication stalling and transcription inhibition in vitro in a repeat-length-dependent manner, and the transcription inhibition could partially contribute to a reduced gene expression in cells. Our results demonstrate that the DNA G-quadruplex of RFC1 PRE participate in aberrant molecular cascades, and provide an unprecedented high-resolution structural target to discover helicases and ligands that resolve the pathogenic G4 for therapeutic intervention.