Distinct eLPB ChAT projections for methamphetamine anxiety and relapse

Wenwen Chen; Hao Guo; Ning Zhou; Xing Xu; Yuning Mai; Teng He; Jun Wen; Feifei Ge; Shan Qin; Chengyong Liu; Wenzhong Wu; Hee Young Kim; Yu Fan; Xiaowei Guan

doi:10.1101/2023.10.23.563030

NeurosciencebioRxivOct 23, 2023

Preprint

Distinct eLPB ChAT projections for methamphetamine anxiety and relapse

Wenwen Chen iD, Hao Guo iD, Ning Zhou, Xing Xu iD, Yuning Mai iD, Teng He, Jun Wen, Feifei Ge iD, Shan Qin iD, Chengyong Liu iD, Wenzhong Wu iD, Hee Young Kim iD, Yu Fan iD, Xiaowei Guan iD

Abstract

Choline acetyltransferase-positive neurons in the external lateral parabrachial nucleus (eLPBChAT) send projections to PKC{delta}-positive (PKC{delta}+) neurons in lateral portion of central nucleus of amygdala (lCeAPKC{delta}) and oval portion of bed nucleus of the stria terminalis (ovBNSTPKC{delta}), forming eLPBChAT-lCeAPKC{delta} and eLPBChAT-ovBNSTPKC{delta} pathways. At least in part, the eLPBChAT neurons positively innervate lCeAPKC{delta} and ovBNSTPKC{delta} through regulating synaptic elements of presynaptic acetylcholine (Ach) release and postsynaptic nicotinic acetylcholine receptors (nAChRs). Methamphetamine (METH) withdrawal anxiety and METH-primed reinstatement of conditioned place preference (CPP) recruit eLPBChAT-lCeAPKC{delta} pathway and eLPBChAT-ovBNSTPKC{delta} pathway in male METH-exposed mice, respectively.

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