Mobilization of genes encoding potential PFAS-degradation enzymes and positive selection in cyanobacteria

Abstract

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental pollutants due to their strong carbon-fluorine bonds and the toxicity of released fluoride from degradation, posing significant challenges for bioremediation. While microbial defluorination of PFAS has been described, the enzymatic mechanisms and evolutionary selection driving this capability is poorly understood. In this study, we screened all complete bacterial genomes in the NCBI RefSeq database for homologs of 43 candidate defluorinating enzymes, focusing on their genetic context and association with mobile genetic elements. Our analysis revealed that fluoroacetate dehalogenases and haloacid dehalogenases are among the most frequently mobilized enzyme classes, suggesting positive selection. Notably, a conserved gene encoding a fluoroacetate dehalogenase homolog in Microcystis cyanobacteria is consistently flanked by genes related to stress response, photosynthesis, and toxin-antitoxin systems, suggesting a potential adaption to alleviate PFAS toxicity. We propose that positive selection for PFAS degradation may be driven by mitigation of physiological stress rather than metabolic gain. These findings highlight the importance of considering ecological and genetic contexts in the search for effective PFAS-degrading microorganisms. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=116 SRC="FIGDIR/small/666183v1_ufig1.gif" ALT="Figure 1"> View larger version (36K): org.highwire.dtl.DTLVardef@9a97f4org.highwire.dtl.DTLVardef@16e3969org.highwire.dtl.DTLVardef@17b2b97org.highwire.dtl.DTLVardef@16adedb_HPS_FORMAT_FIGEXP M_FIG C_FIG