Clemens Mayer

Substituting a conserved histidine alleviates difluoroacetate-dependent isoaspartate formation in a fluoroacetate dehalogenase

This study identifies a conserved histidine residue critical for fluoroacetate dehalogenase activity on difluoroacetate and shows substitutions enhance its catalytic efficiency.

Engineering fluoroacetate dehalogenase by growth-based selections to degrade non-natural organofluorides

This study engineers fluoroacetate dehalogenase (FAcD) variants to degrade non-natural organofluorides using growth-based selections, identifying improved activities and an inhibition pathway.

Pulcherriminic acid biosynthesis and Transport: Insights from a heterologous system in Saccharomyces cerevisiae

The paper characterizes pulcherriminic acid biosynthesis in yeast using a heterologous system, showing that K. lactis genes produce faster acid than Metschnikowia genes and that Pul3 mediates both uptake and export of the compound.

A robust life-or-death selection platform for enzyme evolution

This paper introduces a life-or-death selection platform for enzyme evolution that identifies highly efficient biocatalysts through serial passaging of carbamoylase variants.

Selecting better biocatalysts by complementing recoded bacteria

This paper describes an in vivo selection strategy using recoded bacteria to evolve biocatalysts with improved catalytic efficiency for non-canonical amino acids.