CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies
Moises Di SanteiD, Melissa PezzottiiD, Julius ZimmermanniD, Alessandro EnricoiD, J.R. DeschampsiD, Elisa BalmasiD, Silvia BeccaiD, Eloisa TorchiaiD, Giulia Ballio, Samantha SolitoiD, Martina SarchiiD, Alessandro RealiiD, Alessandro BerteroiD, Florian JugiD, Francesco S. PasqualiniiD
Abstract
Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.
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