Alessandro Bertero

Genetic control couples TGFβ-independent pluripotent stem cell renewal to deterministic forward programming across germ layers

This paper introduces GERALT, a genetic circuit that enables TGFβ-independent hiPSC renewal and inducible lineage programming, improving differentiation efficiency and reducing medium complexity.

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

CALIPERS enables cell cycle-aware live imaging by integrating a modified FUCCI variant, analysis software, and reporter lines for phenotyping and regeneration studies.

Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement via Single-Cell Genomics

The paper introduces CIRI, a CRISPR-based platform for programmable cell fate conversion in hiPSCs, using single-cell genomics to identify regulatory factors for myogenic differentiation.

Prediction and functional interpretation of inter-chromosomal genome architecture from DNA sequence with TwinC

TwinC predicts trans-chromosomal DNA contacts using DNA sequence and provides insights into their functional mechanisms.

Opposing CTCF and GATA4 activities set the pace of chromatin topology remodeling during cardiomyogenesis

The paper shows that CTCF and GATA4 have opposing roles in chromatin remodeling during cardiomyogenesis, with CTCF inhibiting and GATA4 promoting topological changes.

Nascent transcript O-MAP reveals the molecular architecture of an RBM20-regulated single-locus subnuclear compartment organized around the TTN RNA

The paper uses O-MAP to study a cardiac-specific RNA factory around TTN RNA, revealing its chromatin interactions, RNA composition, and role of RBM20 in gene regulation.

Systematic identification of inter-chromosomal interaction networks supports the existence of RNA factories

The paper introduces a computational method to identify trans-chromosomal interactions and finds that genes regulated by the same factor co-localize to form RNA factories.

Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation

This study investigates how 3D chromatin organization changes during human endothelial cell differentiation and links these changes to gene expression and transcriptional regulation.

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

This study identifies TBXT's role in early posterior regionalisation of human neural crest cells through chromatin remodelling and WNT signalling.

Chromatin compartment dynamics in a haploinsufficient model of cardiac laminopathy

The paper investigates how lamin A/C haploinsufficiency affects chromatin compartments and gene expression in human cardiomyocytes, finding limited compartmental changes despite electrophysiological and gene expression alterations.

Dynamic reorganization of nuclear architecture during human cardiogenesis

The paper studies how nuclear architecture changes during human heart development using Hi-C, RNA-seq, and ATAC-seq, revealing dynamic chromosomal organization and gene regulation.