One-pot enzymatic synthesis of the sugar nucleotide CDP-ribitol
Saeed AkkadiD, Eva Wu-Di WaniD, Alex MaddisoniD, Jessica Rule Mcloughin, Cindy Au-Yeung, Lloyd D. MurphyiD, Greg Laughlan McNeiliD, Lianne I. WillemsiD
Abstract
CDP-ribitol serves as an essential donor for D-ribitol-5-phosphate incorporation into bacterial and mammalian glycoconjugates. Here, we demonstrate the one-pot enzymatic synthesis of CDP-ribitol from the readily available precursor ribitol. We also explore the synthesis of bioorthogonally tagged derivatives and other CDP conjugates, thereby providing valuable new tools for glycoscience research.
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