Physiology and lipid accumulation capacity of different Yarrowia lipolytica and Rhodosporidium toruloides strains on glycerol

Abstract

ObjectiveTo compare physiological and process parameters, as well as lipid accumulation capacity, of six strains of Yarrowia lipolytica and two strains of Rhodosporidium toruloides in media containing glycerol as the main carbon and energy source.\n\nResultsThe strains Y. lipolytica IMUFRJ 50678, Po1g, W29 and CCT 5443 displayed very similar physiological parameters, with {micro}max, [Formula] and [Formula] average values of 0.31 h-1, 0.53 (g DW/g S), 0.60 (g S/g DW.h) and 0.46 (g DW/g S), respectively. The two strains of R. toruloides presented physiological and process parameters with no significant difference, with average values of 0.084 h-1, 0.53 (g DW/g S), 0.17 (g S/g DW.h) and 0.44 (g DW/g S). Among all Y. lipolytica strains, Y. lipolytica CCT 5443 strain presented the highest YLip/S, 0.054 (g Lip/g S), and PLipof 0.040 (g Lip/l.h). Among all investigated strains (Y. lipolytica and R. toruloides), the yeast R. toruloides CCT 7815 displayed the highest lipid accumulation capacity, with YLip/Sequal to 0.11 (g Lip/g S) and PLipequal to 0.10 (g Lip/l.h).\n\nConclusionAmong all strains investigated in our study, the yeast strain R. toruloides CCT 7815 presents the most promising characteristics for industrial single cell oil production.\n\nList of abbreviations