bioRxivBioengineering
A mutant of the phototoxic protein KillerRed that does not form DsRed-like chromophore
Abstract
We report a mutant of the phototoxic fluorescent protein KillerRed protein with fluorescence in the green part of the spectrum. The mutant variant carries mutations I64L, D114G, and T115S and does not form a DsRed-like chromophore. The protein can be used as a template to create new genetically encodable photosensitizers that are spectrally different from KillerRed.
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