damidBind: an R/Bioconductor package for differential DamID analysis and data exploration

Abstract

SummaryDamID, and its cell-type specific adaptations, including Targeted DamID (TaDa) and Chromatin Accessibility TaDa (CATaDa), are now widely-adopted as techniques for the genome-wide profiling of DNA binding proteins. Despite this popularity, no dedicated software solution exists for identifying differentially bound or accessible loci, or differentially transcribed genes, between cell types using DamID. The R/Bioconductor package damidBind provides these functions, allowing an end-user to move from processed binding profiles to identifying differentially-bound loci in a reproducible, statistically appropriate and straightforward workflow. Availability and ImplementationdamidBind is an open-source R/Bioconductor package and freely available from Bioconductor at https://bioconductor.org/packages/-damidBind/, and from GitHub at https://github.com/marshall-lab/damidBind. It is released under the GPLv3 licence. ContactOwen Marshall (owen.marshall@utas.edu.au)