Plant tissue culture and regeneration

A Simple and Scalable Chopped-Thallus Transformation Method for Marchantia polymorpha

This paper introduces a new method for transforming Marchantia polymorpha using chopped thalli, achieving high efficiency and scalability for genetic studies.

Shoot at Site: Advancing in planta transformation, regeneration and gene-editing through a cascade of wounding-mediated developmental regulators

This paper develops a synthetic system using wound-induced regulators to regenerate transgenic and gene-edited shoots in planta, bypassing traditional tissue culture methods.

Multiomics-based assessment of the impact of airflow on diverse plant callus cultures

The paper assesses airflow's impact on plant callus cultures using multiomics data from tobacco, rice, and bamboo species, revealing species-specific and within-species differences.

Model-based fed-batch cultivation of Viola odorata plant cells exhibiting antimalarial and anticancer activity

This paper develops a fed-batch cultivation method for Viola odorata plant cells, achieving higher biomass production and demonstrating antiplasmodial activity comparable to natural extracts.

New integrative vectors increase Agrobacterium rhizogenes transformation and help characterise roles for soybean GmTML gene family members

The paper develops new integrative vectors for Agrobacterium rhizogenes transformation, improving efficiency and enabling functional analysis of soybean GmTML gene family members.

From Pigments to Precision: Exploring Genetic Transformation and Genome Editing in Wheat and Barley

This study uses genetic tools to improve wheat and barley transformation and gene editing, showing both benefits and limitations of different approaches.

Double haploid development in drug-type Cannabis sativa L. through microspore indirect de-novo plant regeneration.

This study reports successful double haploid induction in Cannabis sativa through anther culture and indirect de-novo organogenesis, with 29.48% callus induction success and mixoploidy observed.

Optimisation of explant-specific isolation, culture, and micro-callus induction of sesame (Sesamum indicum L.) protoplasts

This study optimizes the isolation and culture of sesame protoplasts from different explants to improve yield and viability for biotechnological applications.

Floral Reversion based micropropagation of Day-Neutral Cannabis sativa L.

This study demonstrates that floral reversion can be used to clonally propagate day-neutral Cannabis sativa plants by optimizing photoperiod conditions.

The H3K27me3 epigenetic mark is crucial for callus cell identity and for the acquisition of new fate during root and shoot regeneration

The paper investigates the role of H3K27me3 in maintaining chromatin accessibility of developmental genes in Arabidopsis callus cells, showing its necessity for regeneration and fate acquisition.

Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

This paper introduces Wuschel2 to enhance CRISPR/Cas genome editing efficiency in sorghum by improving regeneration and reducing tissue culture time.

Establishment of an efficient transformation and CRISPR/Cas9-mediated gene editing system in Chinese local planting cassava (Manihot esculenta Crantz) cultivar SC8

The paper establishes an efficient Agrobacterium-mediated transformation system and CRISPR/Cas9 gene editing in cassava SC8, achieving high transgenic line production and homozygous mutations.

Development of transient expression assay for Cannabis sativa which revealed differential Agrobacterium susceptibility among cannabis cultivars

In vitro induced floral reversion in switchgrass (Panicum Virgatum L.)

The paper describes an in vitro method to induce floral reversion in switchgrass, generating shoots from flower-derived tissues.

Widespread genome instability in Solanum tuberosum plants regenerated from protoplasts

The paper analyzes genome instability in potato plants regenerated from protoplasts, finding widespread aneuploidy and chromosomal segmental changes.

RepB C-terminus mutation of an ori pRi vector affects plasmid copy number in Agrobacterium and transgene copy number in plants

Endophyte Chaetomium globosum D38 and its elicitors promote tanshinones accumulation of Salvia miltiorrhiza

This study shows that the endophytic fungus Chaetomium globosum D38 and its extracts increase tanshinone production in Salvia miltiorrhiza hairy roots and promote plant growth.