Plant tissue culture and regeneration

An in vitro regeneration system with efficient rooting in sweet orange (Citrus sinensis) supports recovery of transgenic plants

This paper develops an efficient in vitro regeneration system for sweet orange that improves rooting and transgenic plant recovery.

STM is required for fate establishment of productive shoot progenitor cells in Arabidopsis tissue culture

This study identifies STM as a key regulator for the transition of shoot progenitor cells to productive shoot progenitor cells in Arabidopsis tissue culture.

Tricotyledonous Seedling in Jute (Corchorus capsularis): First Observation During Tissue Culture Selection

A tricotyledonous seedling was observed in white jute during tissue culture, marking the first documented occurrence of this trait in the cultivar JRC321.

Membrane systems for lipid secretion in the hairy roots of Lithospermum erythrorhizon observed by electron microscopy

This study uses electron microscopy to show that multivesicular bodies mediate both synthesis and secretion of shikonin derivatives in hairy roots of Lithospermum erythrorhizon.

Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing

This study develops a method using developmental regulators to improve soybean transformation and CRISPR-mediated genome editing efficiency.

Relationship between concentration of cytokinin for efficient shoot regeneration and seed coat color in leaf lettuce

The paper shows that BAP concentration needed for efficient shoot regeneration in leaf lettuce varies between white and brown seed cultivars.

Trichome formation in Nicotiana benthamiana is induced by Agrobacterium

The paper shows that certain Agrobacterium strains induce dense glandular trichome formation in Nicotiana benthamiana leaves, attributed to the presence of a trans-zeatin synthase gene.

Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis

This study enhances Agrobacterium-mediated genome editing in Arabidopsis by optimizing floral stages and engineering Agrobacterium to evade plant immunity, achieving high transformation efficiency without antibiotic selection.

Cytokinin-mediated trichome initiation in Nicotiana benthamiana upon Agrobacterium tumefaciens infiltration

This study shows that Agrobacterium strains producing cytokinins induce trichome initiation and acylsugar production in N. benthamiana, highlighting cytokinin's role in trichome development and potential experimental artifacts.

Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdoms

This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.

Phytochemicals and Antioxidant Activity of Leaf Extract and Callus Cultures of Cinnamomum camphora L.

This study compares phytochemicals and antioxidant activity between callus cultures and leaf extracts of Cinnamomum camphora, finding higher antioxidant enzymes and phenolics in callus cultures.

Establishment of an in vitro Culture and Regeneration Protocol for the Native Grass Polypogon australis Brong.

This paper establishes an in vitro regeneration protocol for the native grass Polypogon australis, enabling large-scale propagation and genetic transformation.

Mammalian growth-regulating factors enhance regeneration of recalcitrant transgenic tomato accessions

The paper shows that adding mammalian growth factors to transformation medium improves regeneration rates in recalcitrant tomato accessions.

Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies for Spirodela polyrhiza (Greater Duckweed)

This study optimizes in vitro transformation protocols for Spirodela polyrhiza, achieving high transformation efficiencies and rapid regeneration of genetically modified plants.

Just add water: A simple floral bud injection method for stable Agrobacterium-mediated transformation in two ecotypes of Mimulus guttatus

The paper introduces a floral injection method to improve Agrobacterium-mediated transformation in Mimulus guttatus, enabling transformation of both coastal perennial and inland annual ecotypes.

Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.)

This study demonstrates that viral vectors can enhance leaf transformation in Sorghum bicolor by delivering morphogenic regulators, achieving higher embryonic calli formation rates than conventional vectors.

Efficient and Versatile Rapeseed Transformation for New Breeding Technologies

The paper develops an efficient transformation method for winter-type rapeseed using WUSCHEL from Beta vulgaris, enabling CRISPR/Cas9 editing of gene families and phenotypic analysis.

The Q-Warg Pipeline: A Robust and Versatile Workflow for Quantitative Analysis of Protoplast Culture Conditions

The Q-Warg pipeline quantitatively compares protoplast culture conditions to optimize cell wall recovery and viability, offering a user-friendly tool for unbiased analysis.

Gene regulatory network analysis of somatic embryogenesis identifies morphogenic genes that increase maize transformation frequency

The paper identifies morphogenic genes that enhance maize transformation frequency by analyzing gene regulatory networks in induced somatic embryos.

Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato

This study shows that expressing a GRF-GIF chimera in tomato accelerates shoot regeneration and increases transgenic plant recovery, improving genome editing efficiency.

Single-cell mass spectrometry reveals heterogeneous triterpenic acid accumulation in apple callus-derived cells

This study uses single-cell mass spectrometry to reveal metabolic heterogeneity in apple callus cells and shows that abiotic stress increases triterpenic acid production.

Simple, fast, low-cost method to produce transgenic pollen

This paper develops a low-cost method to deliver genes into plant pollen using needle-free jet injection and vacuum infiltration, demonstrating its effectiveness in multiple plant species without tissue culture.

Optimization of a Cannabis sativa micropropagation protocol (chemotype III) to preserve its cannabinoid profile

This study optimizes a micropropagation protocol for Cannabis sativa (chemotype III) to maintain its CBD/{Delta}9-THC profile, finding that TDZ and BAP treatments affect growth parameters differently over time.

Effect of plant tissue culture parameters on the ploidy level of Physalis grisea, Solanum lycopersicum, and Solanum prinophyllum regenerants

The paper investigates how plant tissue culture parameters affect polyploidy in three Solanaceae species, finding that explant type and species influence polyploidy rates, but zeatin concentration has species-specific effects.

ESR2 orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation

This study shows ESR2 promotes cytokinin biosynthesis and signaling inhibition, which together drive green callus formation in plants.

Treatment with Metal-Organic Frameworks (MOFs) elicits secondary metabolite accumulation in Aquilaria crassna (Agarwood) callus culture

The paper investigates using metal-organic frameworks (MOFs) to elicit secondary metabolite accumulation in Aquilaria crassna callus cultures, finding that certain MOFs significantly increase metabolite production.

Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation

This paper presents a semi-automated protocol for Agrobacterium-mediated plant transformation in Marchantia polymorpha, improving efficiency and reducing costs for high-throughput experiments.

DEVELOPMENT OF PROTOCOLS FOR THE CULTIVATION OF WORMWOOD (ARTEMISIA SALSOLOIDES)

The paper develops and optimizes nutrient media for cultivating Artemisia salsoloides and identifies key genes involved in artemisinin-like compound synthesis.

DEVELOPMENT OF PROTOCOLS FOR DEPOSITING A RARE PLANT SPECIES OF THE ROSTOV REGION, THYMUS CALCAREUS KLOKOV & DES. SHOST.

This paper develops a protocol for in vitro propagation of the rare plant Thymus calcareus, identifying optimal sterilization, nutrient media, and growth regulator conditions for multiplication and rhizogenesis.

Study of the molecular and spatial structure of trans-cinnamic acid isolated from the hairy root culture of Scutellaria baicalensis

This study confirms the molecular structure of trans-cinnamic acid isolated from Scutellaria baicalensis hairy root culture using diffraction analysis and NMR spectroscopy.