Plant tissue culture and regeneration

Micropropagation and Assessment of Genetic Homogeneity in Dendrobium moniliforme (L.) Sw. using Molecular Markers

This study develops a micropropagation protocol for Dendrobium moniliforme and confirms genetic stability using RAPD and ISSR markers.

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon

The paper uses CRISPR/Cas9 to edit the LePPO1 gene in Lithospermum erythrorhizon, showing it is involved in shikonin biosynthesis and explains the role of copper in the process.

Optimization of Agrobacterium-mediated transformation of commercial heirloom tomato cultivars to develop novel traits via CRISPR/Cas9 Genome Editing

The paper optimizes Agrobacterium-mediated transformation in heirloom tomato cultivars to enable CRISPR/Cas9 genome editing for trait improvement.

Improving Perennial Ryegrass Transformation Protocols with Developmental Regulators

This study improves perennial ryegrass transformation by using developmental regulator genes, enabling efficient plant regeneration across genotypes.

From Metabolomics to Function: Ranking Plant Stem Cell Metabolomes for Use in Health and Cosmetics

The paper ranks plant callus metabolomes for health and cosmetic applications by identifying functionally relevant metabolites using a new pipeline.

Vacuum and sonication treatment enables efficient transient gene expression in various monocot and eudicot plant seedlings

This paper introduces VAST, a method improving transient gene expression in plant seedlings, showing enhanced efficiency across multiple species.

Controlling GRF4-GIF1 Expression for Efficient, Genotype-Independent Transformation Across Wheat Cultivars

This study develops an optimized Agrobacterium-mediated wheat transformation protocol using GRF4-GIF1, enabling efficient, genotype-independent transformation across multiple wheat cultivars.

Stable Inheritance of Transgene and Yellow Fluorescent Protein Gene Expression in Progeny of Transgenic Cacao (Theobroma cacao) Plants

The paper demonstrates stable transgene and YFP expression inheritance in cacao progeny using transgenic plants as pollen or ovule donors.

Choosing the Best Route: Comparative Optimization of Wheat Transformation Methods for Improving Yield by Targeting TaARE1-D with CRISPR/Cas9

The paper optimizes three wheat transformation methods using CRISPR/Cas9 to target TaARE1-D, achieving high transformation efficiencies and improving yield traits in wheat mutants.

Strain, procedures, and tools for reproducible genetic transformation and genome editing of Spirodela polyrhiza (L.) Schleid.

This paper identifies a strain of Spirodela polyrhiza suitable for genetic transformation and genome editing, provides detailed procedures, and offers tools for genome exploration and CRISPR design.

An in vitro regeneration system with efficient rooting in sweet orange (Citrus sinensis) supports recovery of transgenic plants

This paper develops an efficient in vitro regeneration system for sweet orange that improves rooting and transgenic plant recovery.

STM is required for fate establishment of productive shoot progenitor cells in Arabidopsis tissue culture

This study identifies STM as a key regulator for the transition of shoot progenitor cells to productive shoot progenitor cells in Arabidopsis tissue culture.

Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing

This study develops a method using developmental regulators to improve soybean transformation and CRISPR-mediated genome editing efficiency.

Relationship between concentration of cytokinin for efficient shoot regeneration and seed coat color in leaf lettuce

The paper shows that BAP concentration needed for efficient shoot regeneration in leaf lettuce varies between white and brown seed cultivars.

Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis

This study enhances Agrobacterium-mediated genome editing in Arabidopsis by optimizing floral stages and engineering Agrobacterium to evade plant immunity, achieving high transformation efficiency without antibiotic selection.

Phytochemicals and Antioxidant Activity of Leaf Extract and Callus Cultures of Cinnamomum camphora L.

This study compares phytochemicals and antioxidant activity between callus cultures and leaf extracts of Cinnamomum camphora, finding higher antioxidant enzymes and phenolics in callus cultures.

Just add water: A simple floral bud injection method for stable Agrobacterium-mediated transformation in two ecotypes of Mimulus guttatus

The paper introduces a floral injection method to improve Agrobacterium-mediated transformation in Mimulus guttatus, enabling transformation of both coastal perennial and inland annual ecotypes.

Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.)

This study demonstrates that viral vectors can enhance leaf transformation in Sorghum bicolor by delivering morphogenic regulators, achieving higher embryonic calli formation rates than conventional vectors.

Efficient and Versatile Rapeseed Transformation for New Breeding Technologies

The paper develops an efficient transformation method for winter-type rapeseed using WUSCHEL from Beta vulgaris, enabling CRISPR/Cas9 editing of gene families and phenotypic analysis.

The Q-Warg Pipeline: A Robust and Versatile Workflow for Quantitative Analysis of Protoplast Culture Conditions

The Q-Warg pipeline quantitatively compares protoplast culture conditions to optimize cell wall recovery and viability, offering a user-friendly tool for unbiased analysis.

Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato

This study shows that expressing a GRF-GIF chimera in tomato accelerates shoot regeneration and increases transgenic plant recovery, improving genome editing efficiency.

Single-cell mass spectrometry reveals heterogeneous triterpenic acid accumulation in apple callus-derived cells

This study uses single-cell mass spectrometry to reveal metabolic heterogeneity in apple callus cells and shows that abiotic stress increases triterpenic acid production.

ESR2 orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation

This study shows ESR2 promotes cytokinin biosynthesis and signaling inhibition, which together drive green callus formation in plants.

Treatment with Metal-Organic Frameworks (MOFs) elicits secondary metabolite accumulation in Aquilaria crassna (Agarwood) callus culture

The paper investigates using metal-organic frameworks (MOFs) to elicit secondary metabolite accumulation in Aquilaria crassna callus cultures, finding that certain MOFs significantly increase metabolite production.

Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation

This paper presents a semi-automated protocol for Agrobacterium-mediated plant transformation in Marchantia polymorpha, improving efficiency and reducing costs for high-throughput experiments.

A Simple and Scalable Chopped-Thallus Transformation Method for Marchantia polymorpha

This paper introduces a new method for transforming Marchantia polymorpha using chopped thalli, achieving high efficiency and scalability for genetic studies.

Multiomics-based assessment of the impact of airflow on diverse plant callus cultures

The paper assesses airflow's impact on plant callus cultures using multiomics data from tobacco, rice, and bamboo species, revealing species-specific and within-species differences.

Model-based fed-batch cultivation of Viola odorata plant cells exhibiting antimalarial and anticancer activity

This paper develops a fed-batch cultivation method for Viola odorata plant cells, achieving higher biomass production and demonstrating antiplasmodial activity comparable to natural extracts.

New integrative vectors increase Agrobacterium rhizogenes transformation and help characterise roles for soybean GmTML gene family members

The paper develops new integrative vectors for Agrobacterium rhizogenes transformation, improving efficiency and enabling functional analysis of soybean GmTML gene family members.

Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

This paper introduces Wuschel2 to enhance CRISPR/Cas genome editing efficiency in sorghum by improving regeneration and reducing tissue culture time.