Integrating Genetic Domestication and Model-Guided Kill-Switch Engineering in a Non-Model Bacterial Therapeutic
Yeonhwee Jeong, Hari R. Namboothiri, Oluwatola Femi-Olatunji, Paul de Figueiredo, Cansu Agca, Chelsea Y. Hu
Host-measured parts and inducible systems for Brucella melitensis ΔvjbR guided a modeled Cas9 kill-switch, which cut mouse liver bacterial burden by about 91% at 30 mg/kg doxycycline.
bioRxiv · Oct 20, 2026 · PreprintSynthetic Biology✦ In-depth digest