Hari R. Namboothiri

Host-measured parts and inducible systems for Brucella melitensis ΔvjbR guided a modeled Cas9 kill-switch, which cut mouse liver bacterial burden by about 91% at 30 mg/kg doxycycline.

The paper develops growth-aware optogenetic control strategies to maintain gene expression in bacterial batch cultures under population-level disturbances.

This paper describes the fabrication and use of a 32-well LED-embedded microplate (LEMOS 2.0) for optogenetic stimulation and gene expression measurement in microbial cultures.

This paper introduces LEMOS, a low-cost microplate reader for optogenetic feedback control in batch cultures, demonstrating closed-loop gene expression tracking and growth-aware controller design.

This paper develops a multiscale model, GEAGS, to predict transient oscillations in synthetic gene circuits by coupling gene expression to growth phases, revealing amino acid recycling and growth transitions as key factors.