Steroid Chemistry and Biochemistry

Complete genome sequence of Comamonas testosteroni TA441 and comparative analysis of steroid degradation gene clusters across Proteobacteria and Actinomycetota

Biochemical characterization of bacterial methyltransferases reveals necessary residues for sterol side-chain propylation

The paper identifies key residues in bacterial SMTs necessary for sterol side-chain propylation and shows their conservation across methylating, ethylating, and propylating SMTs.

The urinary pathobiont Actinobaculum massiliense generates androgens via the dirAB pathway

The study identifies Actinobaculum massiliense as a urinary pathobiont capable of generating androgens via the dirAB pathway, using a novel HSDH assay.

Characterization of an NADPH-dependent 17α-hydroxysteroid dehydrogenase encoded by the desF gene from the gut bacterium Clostridium scindens VPI 12708

This study quantitatively characterizes the kinetic parameters and substrate specificity of the NADPH-dependent 17α-hydroxysteroid dehydrogenase encoded by the desF gene in Clostridium scindens VPI 12708, revealing its role in epitestosterone formation.

Before the Black Box

The paper investigates evolutionary changes in three enzymes involved in ecdysteroid synthesis across arthropods and correlates these changes with dietary factors.

Identification of Dehydrogenase, Hydratase, and Aldolase Required for C17 Propionyl Residue Removal in Steroid Degradation by Comamonas testosteroni TA441: Structural and Functional Insights into the (ChsE1-ChsE2)2 and (ChsH1-ChsH2MaoC)2-(Ltp2-ChsH2DUF35)2 Complexes

This study identifies enzymes involved in C17 propionyl residue removal during steroid degradation in Comamonas testosteroni TA441 and shows structural and functional similarities to Mycobacterium tuberculosis enzymes.