Discover, rate, and discuss biology preprints.

This study uses single-molecule FRET to show that gp41-directed antibodies stabilize or open the prefusion-closed state of HIV-1 Env, revealing two neutralization mechanisms.

The paper reports early surveillance in Australia's Murray-Darling Basin detecting two LPAI viruses in wild waterbirds, aiding in preparedness for potential H5N1 outbreaks.

The paper identifies Pph22, Tap42, and Rrd2 as components of the protein phosphatase 2A complex in Cryptococcus neoformans, showing their roles in stress response, virulence, and morphogenesis.

The paper investigates redox distribution of Asgard archaea and co-occurring taxa in microbial mats from an early Proterozoic ecosystem analog, revealing their ecological niches and potential roles in early eukaryogenesis.

This study identifies Sig7-8 as a two-subunit sigma factor in Tn916 that activates transcription but causes cell death, and shows that gene expression from Psig7 is limited in transconjugants.

This study tracks rhizosphere microbiome assembly in tomato plants during early development, showing inoculum-constrained succession with increased phylogenetic clustering and diversity.

The paper identifies a novel virus lineage, IME1, found in Dehalococcoides-containing cultures, characterized by features similar to filamentous bacterial viruses.

This study evaluates start-up and stabilisation dynamics in five parallel CSTR biogas systems, finding that microbial communities diverge from the original inoculum and require extended stabilisation.

This study evaluates the replication of human norovirus in salivary gland cells and finds limited replication of certain isolates despite testing various supplements and inhibitors.

This study examines how Phytophthora cinnamomi affects avocado rhizosphere fungal communities, finding shifts in composition and potential antagonists.

This study shows that domesticated plasmids in Pantoea agglomerans enhance bacterial fitness and metabolic integration with the chromosome during adaptation to a plant-associated lifestyle.

This study investigates MOMP assembly in Corynebacterium glutamicum, showing that PorH and ProtX are inserted at polar growth zones and linked to the cell wall.

This study compares antibiotic resistance development in E. coli across LB broth, synthetic wastewater, and real wastewater, finding synthetic wastewater better predicts growth and resistance than LB.

This study evaluates intranasal adenovirus type 5 influenza vaccination in pregnant and lactating ferrets, finding it enhances milk antibody responses and passive transfer to neonatal offspring.

A defined rumen microbial consortium reduced culling risk in dairy cattle across multiple trials, with consistent effects on health outcomes.

Cache sensor domains of DUF3365/Tll0287 family bind covalent c-type heme through a CXXCH insertion; a signaling member behaves like an NO sensor, a stand-alone one like a redox protein.

This paper presents an optimized DNA extraction protocol for adhesive samplers to enable culture-independent ITS2-based metabarcoding of fungal bioaerosols, revealing site-specific and seasonal fungal community dynamics.

This study shows that elderly individuals vaccinated against SARS-CoV-2 maintain neutralizing antibodies against recent variants, with reduced immune imprinting from ancestral variants after booster doses.

The paper investigates how human-targeted drugs alter regulatory networks in Salmonella Typhimurium, revealing non-antibiotic compounds reshape virulence and antibiotic resistance through specific transcriptional responses.