Discover, rate, and discuss biology preprints.

CNN patch classifiers trained only on normal breast H&E slides label epithelium, stroma and adipocytes with AUCs of 0.98–1.00 on three external cohorts and feed a lobule-detection pipeline.

Cyclic immunofluorescence of 265 high-grade serous ovarian carcinomas and random-forest feature ranking identify cancer-cell MHC-II as an adjusted predictor of longer survival marking immune-activated tumor-stroma hotspots.

Paired CSF and blood scRNA/TCR-seq in treatment-naive MS shows a small set of expanded, cytotoxic CSF-enriched CD8+ clonotypes, two of which recognize EBV epitopes.

DNA origami particles displaying eOD-GT8 with a synthetic T helper epitope gave antigen-focused germinal centers and expanded VRC01-class precursors in humanized mice after one immunization.

Molecular screening of bladder urine from 567 asymptomatic dogs and cats across seven Thai provinces detected pathogenic Leptospira in 9.8% of animals, including the first animal detection of Leptospira yasudae.

Whole-genome sequencing and geolocation tracking indicate that high pathogenicity avian influenza H5N1 reached Tristan skuas on remote Gough Island via sub-Antarctic marine networks linked to South Georgia rather than Africa.

In musical dyads, perturbing breathing disrupts both respiratory and auditory-motor synchronization whereas perturbing tapping disrupts only behavior, demonstrating a unidirectional causal influence from respiration to behavioral coordination.

Morphometric and micro-CT analysis of 110 fossil and 354 extant Solanaceae seeds identifies 10 specimen-backed fossil species, including four new genera and a fossilized elaiosome, spanning Eocene to Holocene deposits.

In 14 rats doing probabilistic reversal learning, model-derived uncertainty (unsigned average prediction error) and recency-weighted reward history distinctly and sex-specifically shaped win-stay versus lose-shift choices.

Wheat IDD5 and barley SDW3, orthologous zinc-finger transcription factors, bind DELLA proteins and promote gibberellin (GA)-driven elongation; their loss-of-function mutants are GA-insensitive semi-dwarfs resembling Rht-D1b.

Single-cell and spatial maps of human coronary arteries identify FAP as a marker of SMC-derived modulated cells; an anti-FAP BiTE reduced plaque burden in mouse models.

A BERT classifier on titles and abstracts flagged 9.87% of 2.6 million cancer papers (1999 to 2024) as resembling retracted paper mill papers, with yearly shares rising past 15%.

FoldMason uses Foldseek's 3Di+AA alphabet for progressive multiple structure alignment, beating structure aligners' LDDT on AlphaFold DB clusters at near sequence-aligner speed, scaling to 100,000 structures.

Orally administered β-1,3- and α-1,4-linked polysaccharides cross intestinal epithelium into rodent plasma and liver as macromolecules via clathrin-, dynamin 1-, and Rab5-dependent endocytosis mediated by EGFR, Dectin-1, and BMPRIA.

Sixteen weeks of heavy resistance exercise in older men produces true inter-individual variability, yet multi-outcome classification shows 82% respond robustly or excellently and only 5% respond poorly.

Real rummy-nose tetras follow a projected, anamorphically rendered virtual conspecific in a low-cost open-source 3D-tracked VR bowl, largely matching its speed and depth; a moving sphere elicits weak following.

For species with 20 or fewer occurrence records, 24 of 31 species distribution models had AUC distributions not significantly different from Maxnet's; vote-counting ensembles give upper and lower range bounds.

In vivo lineage tracing and targeted ablation in autochthonous mouse lung adenocarcinoma establish the Slc4a11+ high-plasticity cell state as a bidirectional transition hub driving tumor progression and therapy resistance.

Whole-genome sequencing of 127 adult Dirofilaria immitis from four continents reveals continental population structure predating dog domestication, an Asian source for Australian worms, and recent Europe-Central America spread.

Cryo-ET of Langat virus-infected cells shows replication organelle size set by a membrane coat plus RNA pressure, with virion budding and maturation occurring beside the organelles.