A. Diaspro

DEK oncoprotein participates in heterochromatin replication via SUMO-dependent nuclear bodies

This study identifies DEK bodies involved in heterochromatin replication and links DEK to SUMO regulation, highlighting its role in chromatin integrity and cancer.

Super-resolution microscopy reveals the nanoscale organization of the DEK protein cancer biomarker

This study uses super-resolution microscopy to analyze DEK protein organization in breast cancer cells, showing cluster features correlate with cell type and chromatin state.

Strategies and experimental tips for optimized quantitative single-molecule studies of membrane and membrane-associated proteins

The paper provides strategies and experimental tips for optimizing quantitative single-molecule studies of membrane and membrane-associated proteins to avoid artifacts and improve accuracy.

Optical-quality assessment of a miniaturized intraocular telescope

The paper assesses the optical quality of a miniaturized intraocular telescope, finding it has uniform transmission and minimal aberrations, making it suitable for imaging in age-related macular degeneration.

Nanoscale engagement and clusterization of Programmed death ligand 1 (PD-L1) in the membrane lipid rafts of Non-Small Cell Lung Cancer cells

This study uses super-resolution microscopy to show PD-L1 clusters in lipid rafts of NSCLC cells, suggesting new immuno-oncology targets.

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy

The paper introduces focus-ISM, a method combining STED and ISM to improve super-resolution microscopy for live and thick samples without compromising image quality.

EVALUATION OF STED SUPER-RESOLUTION IMAGE QUALITY BY IMAGE CORRELATION SPECTROSCOPY (QuICS)

The paper introduces an ICS-based algorithm to evaluate STED image quality, finding that increasing STED power improves resolution but reduces contrast, while SPLIT methods enhance resolution and contrast.

A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells

The paper uses multi-scale fluorescence microscopy to study SARS-CoV-2 variant entry into Vero E6 cells, finding B.1.1.7 enters faster than its ancestor due to the N501Y mutation.

Electrostatics and Solvation: Essential Determinants of Chromatin Compaction

This paper examines the role of electrostatics and solvation in chromatin compaction, highlighting their importance through computational and experimental analyses.

Nanoscale distribution of nuclear sites analyzed by superresolution STED-ICCS

This paper introduces STED-ICCS, a method combining superresolution microscopy with image cross-correlation spectroscopy to quantify nanoscale spatial distribution of functional nuclear sites in MCF10A cells.

Volumetric Lissajous Confocal Microscopy

This paper introduces volumetric Lissajous confocal microscopy to achieve high-speed 3D imaging with tunable sampling rates and sub-millisecond acquisition times.

Chromatin nanoscale compaction in live cells visualized by acceptor-donor ratio corrected FRET between DNA dyes

The paper corrects FRET measurements for acceptor-donor ratio to study chromatin compaction in live cells and shows rapid decompaction after DNA damage.

Easy Two-Photon Image Scanning Microscopy with SPAD Array and Blind Image Reconstruction

This paper introduces a two-photon image scanning microscopy (2PE-ISM) system that improves optical resolution and image quality without user calibration.

The oncoprotein DEK affects the outcome of PARP1/2 inhibition during replication stress

This study shows that DEK interacts with PAR to influence replication fork progression under stress, affecting PARP1/2 inhibitor efficacy in cancer cells.

Measuring mobility in chromatin by intensity sorted FCS

This paper introduces a method to measure chromatin mobility using intensity-sorted FCS, finding reduced GFP mobility in heterochromatin and differential effects of chromatin compaction on diffusion.

Enigma at the nanoscale: can the NPC act as an intrinsic reporter for isotropic expansion microscopy?

This study evaluates expansion microscopy's isotropy by analyzing nuclear pore complexes, finding uniform accuracy within 20 nm for structural studies.

Photon-separation to enhance the spatial resolution in pulsed STED microscopy

This paper introduces SPLIT-pSTED, a method that enhances spatial resolution in pulsed STED microscopy by separating photons without increasing STED beam intensity.

Image Scanning Microscopy with Single-Photon Detector Array

This paper introduces an improved image scanning microscopy technique using a single-photon detector array, enabling better resolution while maintaining compatibility with existing confocal microscopes and fluorescence spectroscopy methods.