Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of Toxoplasma gondii host colonization
C. J. Wincott, G. Sritharan, H. J. Benns, F. B. Liakath, C. Gilabert-Carbajo, M. Bunyan, A. Fairweather, E. Alves, I. Andrews, L. Game, E. M. Frickel, C. Tiengwe, S. E. Ewald, M. A. Child
The paper develops a CRISPR-based barcoding method for protozoa and applies it to Toxoplasma gondii, showing the blood-brain barrier is not a major bottleneck for colonization.
bioRxiv · Aug 6, 2020Microbiology