A. J. Polino

Immuno-Scanning Electron Microscopy of Islet Primary Cilia

This study uses immuno-SEM to identify specific proteins on primary cilia of mouse and human pancreatic islets, overcoming limitations of conventional imaging methods.

Disrupting actin filaments enhances glucose-stimulated insulin secretion independent of the cortical actin cytoskeleton

The paper shows that disrupting actin filaments increases glucose-stimulated insulin secretion without affecting the cortical actin cytoskeleton.

The mature N termini of Plasmodium effector proteins confer specificity of export

This study shows that precise cleavage by signal peptidase to generate the mature N terminus is necessary for export of PEXEL proteins, while PM V cleavage is not required.

Scanning electron microscopy of human islet cilia

This study uses scanning electron microscopy to characterize the ultrastructure of human islet cilia, revealing preserved subdomains and a novel ciliary ring.

A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin V

The paper identifies a nepenthesin insert essential for plasmepsin V function in malaria parasites and shows it allosterically regulates catalysis.

An essential ER-resident N-acetyltransferase in Plasmodium falciparum

The paper identifies an ER-resident N-acetyltransferase in Plasmodium falciparum essential for parasite growth but not for protein export or acetylation of specific exported proteins.

Assessment of biological role and insight into druggability of the Plasmodium falciparum protease plasmepsin V

This paper validates plasmepsin V as an essential malaria parasite protein by showing its depletion arrests parasite growth and reveals an unexpected role in early development.