Long-term high-yield skeletal muscle stem cell expansion through staged perturbation of cytokine signaling in a soft hydrogel culture platform
A. M. Loiben, K. H. Kim, S. Y. Soueid-Baumgarten, V. M. Aguilar, J. Chin Cheong, R. F. Kopyto, P. Fraczek, E. H. H. Fong, R. Mangal, L. A. Archer, B. D. Cosgrove
This paper develops a method to expand skeletal muscle stem cells long-term using a hydrogel substrate, cytokine cocktail, and staged p38/{beta} inhibition, achieving high yields of functional Pax7+ cells.
bioRxiv · Jun 4, 2020 · PreprintBioengineering