A. P. Arkin

The paper identifies toxic inorganic ions that inhibit sensitive microorganisms and favor Rhodanobacter in a metal-contaminated aquifer.

Dub-seq enables high-throughput overexpression screens in E. coli by combining shotgun expression libraries with DNA barcode sequencing to identify genes with gain-of-function phenotypes.

The paper identifies novel enzymes filling amino acid biosynthesis gaps in bacteria using genome-wide mutant fitness data, revealing previously unknown pathways for methionine, threonine, serine, and histidine synthesis.

The paper validates regulatory predictions from bacterial transcription factors using mutant fitness data, identifying significant cofitness for 158 TFs across 24 bacteria.