Enabling Plasmid-based Expression in Clostridium kluyveri using a Biparental Methylation-Conjugation System
Ethan Agena, Abiali A. Badani, Blake G. Lindner, Ian Mateus Gois, Andrew W. Dempster, Nigel Peter Minton, Radhakrishnan Mahadevan, Christopher E. Lawson
This paper develops a methylation-conjugation system for Clostridium kluyveri to enable plasmid-based genetic modification and demonstrates its use for expressing a fluorescent reporter.
ACS Synthetic Biology · Mar 18, 2026Peer-reviewedBioengineering