Nigel Peter Minton

This paper develops a methylation-conjugation system for Clostridium kluyveri to enable plasmid-based genetic modification and demonstrates its use for expressing a fluorescent reporter.

The paper develops a method to create methylating conjugal donor strains to improve DNA transfer into genetically recalcitrant bacteria by mirroring their native DNA methylation signature.

This paper introduces an inducible CRISPR/Cas9 system for precise, markerless genome editing in Synechocystis sp. PCC 6803, enabling multiplexed edits and efficient segregation of mutants.

This paper develops methods to apply transposon-insertion sequencing in Clostridium autoethanogenum to identify essential genes for growth using carbon monoxide.

The paper introduces a test-driven framework for developing and maintaining genome-scale metabolic models, enabling continuous integration of validation data and improving model reliability.