Minimum inoculum of resistance assay for evaluating for anti-toxoplasmosis compounds that target phenyl alanine tRNA synthetase
Taher Uddin, Payal Mittal, Han Xie, Bruno N. Melillo, Amit Sharma, Arnab Chatterjee, L. David Sibley
The paper develops a Minimum Inoculum for Resistance (MIR) assay to evaluate anti-Toxoplasma gondii compounds, showing that inhibitors of phenylalanine tRNA synthetase require higher parasite inocula to induce resistance compared to DNA synthesis inhibitors.
bioRxiv · Mar 3, 2026 · PreprintMicrobiology