Enzymatic Assembly for CRISPR Split-Cas9 System: The Emergence of a Sortase-based Split-Cas9 Technology
Seyed Hossein Helalat, Helga Thora Kristinsdóttir, Astrid Dolinger Petersen, Rodrigo H. Coronel‐Tellez, Mads Nordlund Boye, Yi Sun
This study introduces a Sortase-based split-Cas9 system for precise genome editing, demonstrating its feasibility in E. coli and human cells with reduced off-target effects.
International Journal of Biological Macromolecules · Feb 27, 2025Peer-reviewedMolecular Biology