B. C. Meyers

Unusual predominance of maintenance DNA methylation in Spirodela polyrhiza

This study shows that Spirodela polyrhiza maintains CG and CHG DNA methylation at repeats without the RdDM pathway or small RNAs, despite losing key methylation genes.

Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function

The paper identifies premeiotic 24-nt phasiRNAs in maize and related species, showing they are distinct from meiotic 24-nt phasiRNAs in biogenesis and function.

Fluorescence hybridization chain reaction enables localization of multiple molecular classes combined with plant cell ultrastructure

This paper adapts hybridization chain reaction (HCR) for plant tissue sections to localize multiple mRNAs and antibodies, demonstrating compatibility with electron microscopy for correlating molecular and ultrastructural data.

The floral development of the allotetraploid Coffea arabica L. correlates with a small RNA dynamic reprogramming.

The paper analyzes small RNA dynamics during floral development in Coffea arabica to identify stage-specific regulatory mechanisms affecting flower development and ripening.

Coffee microsporogenesis and related small interfering RNAs biosynthesis have a unique pattern among eudicots suggesting a sensitivity to climate changes.

The paper studies microsporogenesis and phasiRNA biosynthesis in Coffea arabica, revealing unique patterns and temperature sensitivity in eudicots.

Introduction of Barnase/Barstar in soybean produces a rescuable male sterility system for hybrid breeding

This study introduces a Barnase/Barstar system in soybean to create a male sterility system for hybrid breeding, showing that fertility can be rescued by adjusting gene expression levels.

Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genes

The paper shows that plastid-encoded dsRNA can trigger cytoplasmic gene silencing of nuclear genes through phasiRNA production, enabling traits to function outside the plastid.

A Versatile Enhanced Freeze-Substitution Protocol for Volume Electron Microscopy

The paper introduces an enhanced freeze-substitution protocol that improves heavy metal staining in diverse organisms and tissues for volume electron microscopy.

Phylogenetic analyses of AGO/DCL/RDR proteins in green plants refine the evolution of small RNA pathways

This study performs phylogenetic analyses of AGO/DCL/RDR protein families across 196 green plant species to refine the evolutionary history of small RNA pathways.

Arabidopsis Apoplastic Fluid Contains sRNA- and Circular RNA-Protein Complexes that Are Located Outside Extracellular Vesicles

The apoplast of Arabidopsis leaves contains diverse small and long-noncoding RNAs, including circular RNAs, that are bound to protein complexes and are located outside extracellular vesicles.

Characterization of Heat Responsive microRNAs and Phased Small Interfering RNAs in Reproductive Development of Flax

The paper identifies heat-responsive miRNAs and phasiRNAs in flax reproductive development, revealing novel regulatory elements and their differential expression under heat stress.

Tasselyzer, a machine learning method to quantify maize anther exertion, based on PlantCV

Tasselyzer quantifies maize anther exertion using PlantCV for automated, large-scale analysis, capturing regional differences and demonstrating utility in research and breeding.

24-nt phasiRNAs move from tapetal to meiotic cells in maize anthers

The paper shows 24-nt phasiRNAs move from tapetal cells to meiocytes and other somatic cells in maize anthers.

Automated imaging of duckweed growth and development

The paper develops an automated microscopy method to image duckweed growth and shows sucrose enhances growth rate.

The Streptochaeta genome and the evolution of the grasses

This paper sequences and annotates the Streptochaeta angustifolia genome, revealing insights into grass evolution, gene duplication, and small RNA regulation.

The Evolutionary History of Small RNAs in the Solanaceae

The paper uses over 255 small RNA datasets to characterize the small RNA landscape across the Solanaceae family, revealing dynamic evolutionary patterns and genus-specific similarities.

miRador: a fast and precise tool for the prediction of plant miRNAs

miRador is a tool for plant miRNA prediction that is as precise as existing tools but significantly faster.

Conserved and non-conserved triggers of 24-nt reproductive phasiRNAs in eudicots

The paper identifies conserved and non-conserved triggers of 24-nt phasiRNAs in eudicots, including new triggers and expanded loci in wild strawberry and validated pathways in rose.

A new Spirodela polyrhiza genome and proteome reveal a conserved chromosomal structure with high abundances of proteins favoring energy production

This paper presents a new genome and proteome of Spirodela polyrhiza, revealing conserved chromosomal structure and high abundance of energy-related proteins.

Pre-meiotic, 21-nucleotide Reproductive PhasiRNAs Emerged in Seed Plants and Diversified in Flowering Plants

The paper identifies 21-nt reproductive phasiRNAs in eudicots, showing their emergence in seed plants and loss in some lineages.

A transposon surveillance mechanism that safeguards plant male fertility during stress

This study identifies two Argonaute-like proteins essential for maintaining male fertility in maize under high temperatures by controlling retrotransposon activity.

Ligation bias is a major contributor to nonstoichiometric abundances of secondary siRNAs and impacts analyses of microRNAs

The paper evaluates small RNA library construction methods in plants, showing that ligation bias affects secondary siRNA abundances and microRNA analysis.

Environment-conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers

The paper investigates how the HD-ZIP IV transcription factor mutant ocl4 affects 21-nt phasiRNA accumulation in maize anthers under different environmental conditions, finding that these phasiRNAs buffer development under stress.

Pre-meiotic, 24-nt reproductive phasiRNAs are abundant in anthers of wheat and barley but not rice and maize

This study identifies 24-nt reproductive phasiRNAs in pre-meiotic and meiotic stages of wheat and barley anthers, absent in maize and rice.

Surprising amount of stasis in repetitive genome content across the Brassicales

The paper investigates genome size evolution in Brassicales, finding no correlation between genome size and TE content or significant changes in TE landscapes after WGD.

The commitment of barley microspores into embryogenesis involves miRNA-directed regulation of members of the SPL, GRF and HD-ZIPIII transcription factor families

The paper investigates miRNA regulation in barley microspore embryogenesis, identifying miRNA-directed regulation of SPL, GRF, and HD-ZIPIII transcription factors during the transition to embryogenesis.

Visualizing cassava bacterial blight at the molecular level using CRISPR-mediated homology-directed repair

The paper develops a CRISPR-based tool to visualize Cassava bacterial blight dynamics by inserting a GFP reporter into the susceptibility gene MeSWEET10a.

Quantitative, super-resolution localization of small RNAs with sRNA-PAINT

The paper introduces sRNA-PAINT, a method for high-resolution detection and quantification of small RNAs, and demonstrates its application in maize anthers.

Reproductive phasiRNA loci and DICER-LIKE5, but not microRNA loci, diversified in monocotyledonous plants

The paper characterizes miRNA, siRNA, and phasiRNA diversity across 38 monocot species, finding 21-nt reproductive phasiRNAs are more common in grasses and DCL5 likely originated before grass diversification.

Abundant expression of maternal siRNAs is a conserved feature of seed development

The paper shows that maternal siRNAs are abundant and conserved in seed development across angiosperms, with a maternal bias in endosperm and dependence on RNA Pol IV/RDR2.