B. C. Orsburn

Tenofovir Activation is Diminished in the Brain and Liver of Creatine Kinase Brain-Type Knockout Mice

This paper shows that CKB knockout mice have reduced tenofovir activation in brain and liver, with specific mutations impairing CKB's ability to generate the active metabolite TFV-DP.

Acetic acid is a superior ion pairing modifier for sub-nanogram and single cell proteomics

This paper shows that acetic acid improves peptide signal and proteome coverage in sub-nanogram and single cell proteomics compared to formic acid.

Spatial Heterogeneity of Brain Lipids in SIV-infected Macaques Treated with Antiretroviral Therapy

This study examines spatial lipid changes in SIV-infected macaques on ART, finding region-specific lipid alterations that suggest ongoing bioenergetic disruptions in the brain.

EstG is a novel esterase required for cell envelope integrity

This study identifies EstG, a novel esterase in Caulobacter crescentus that protects against cellular stress by acting on cyclic osmoregulated periplasmic glucans rather than the cell wall.

Diagnostic Ion Data Analysis Reduction (DIDAR) allows rapid quality control analysis and filtering of multiplexed single cell proteomics data

DIDAR filters and analyzes multiplexed single cell proteomics data by identifying diagnostic fragment ions to reduce file size and improve quality control.

Single Cell Proteomics Using a Trapped Ion Mobility Time-of-Flight Mass Spectrometer Provides Insight into the Post-translational Modification Landscape of Individual Human Cells

This paper introduces a single cell proteomics method using trapped ion mobility time-of-flight mass spectrometry, enabling high coverage protein quantification and detection of post-translational modifications in individual human cells.

A simple tool extends TIMSTOF compatibility with historic data processing tools and enables ion mobility-enhanced spectral libraries

The paper introduces a data conversion tool that enables TIMSTOF ion mobility data compatibility with existing proteomics pipelines, improving peptide match confidence using folded ion mobility data.

Aliquoting of isobaric labeling reagents for low concentration and single cell proteomics samples

This paper describes a method to aliquot isobaric labeling reagents for proteomics, reducing costs and extending reagent usability over six months.