Fluorescence-based 3D targeting of FIB-SEM acquisition of small volumes in large samples
P. Ronchi, P. Machado, E. D'Imprima, G. Mizzon, B. T. Best, L. Cassella, S. Schnorrenberg, M. G. Montero, M. Jechlinger, A. Ephrussi, M. Leptin, J. Mahamid, Y. Schwab
The paper presents a method to precisely target FIB-SEM acquisition of fluorescently labeled cells in large samples using fluorescence preservation and confocal imaging.
bioRxiv · Jan 18, 2021Cell Biology