A. Ephrussi

An architectural role of oskar mRNA in granule assembly

This study investigates how oskar mRNA's kissing-loop interaction contributes to RNP granule assembly in Drosophila oocytes, showing its role in condensate formation and posterior RNA localization.

Tropomyosin 1-I/C co-ordinates kinesin-1 and dynein motors during oskar mRNA transport

This study shows Tm1-I/C coordinates kinesin-1 and dynein during oskar mRNA transport by inhibiting kinesin-1 until it is needed in the oocyte.

Stably bound adaptor proteins modulate directionality of RNP transport

This study investigates the role of adaptor proteins in kinesin-1 mediated oskar RNA transport, showing that stably bound aTm1 can mediate transport but with less potency than tethered kinesin-1.

Subcellular spatial transcriptomics identifies three mechanistically different classes of localizing RNAs

The paper identifies three distinct mechanisms of RNA localization in Drosophila follicular epithelium using subcellular spatial transcriptomics.

An RNA-based feed-forward mechanism ensures motor switching in oskar mRNA transport

The paper shows that Staufen and Egl interact to switch motor proteins during oskar mRNA transport in Drosophila oocytes, enabling proper localization.

Liquid-to-solid phase transition of oskar RNP granules is essential for their function in the Drosophila germline

This study shows that oskar RNP granules in Drosophila oocytes undergo a liquid-to-solid phase transition, which is essential for their function in germline formation.

Validation and classification of RNA binding proteins identified by mRNA interactome capture

This study validates and classifies RNA binding proteins using NMR and biochemical methods, finding that some novel RBPs interact weakly with RNA and may bind non-specifically.

Fluorescence-based 3D targeting of FIB-SEM acquisition of small volumes in large samples

The paper presents a method to precisely target FIB-SEM acquisition of fluorescently labeled cells in large samples using fluorescence preservation and confocal imaging.

Staufen2 mediated RNA recognition and localization requires combinatorial action of multiple domains

This study shows that dsRBDs 1-2 of mStau2 are essential for mRNA recognition and function, as demonstrated by rescue experiments in Drosophila oocytes.

The transcriptome-wide landscape and modalities of EJC binding in adult Drosophila

The paper identifies EJC binding modalities in Drosophila by developing a method to isolate RNA-bound EJC complexes, revealing canonical binding sites and their association with developmental transcripts.

Enzymatic production of single molecule FISH and RNA capture probes

The paper describes a method to label DNA oligonucleotides with fluorescent dyes or biotin using terminal deoxynucleotidyl transferase, enabling single molecule FISH and RNA capture.

An RNA-binding tropomyosin recruits kinesin-1 dynamically to oskar mRNPs

The paper shows that DmTropomyosin1-I/C recruits kinesin-1 to oskar mRNPs dynamically during oogenesis, with motor activation linked to spliced RNA elements.