P. Machado

Identification and characterization of new structural scaffolds modulating the activity of Mycobacterium tuberculosis dihydroneopterin aldolase (FolB) in vitro

The paper identifies and characterizes new structural scaffolds that modulate the activity of Mycobacterium tuberculosis dihydroneopterin aldolase (FolB) in vitro, finding compounds with IC50 values ranging from 2.6 to 47 μM.

Shotgun proteomic analysis of the caterpillar Lonomia obliqua (Lepitoptera, Saturniidae) hemolymph and effects in rat hippocampal neurons culture

This study identifies neuroprotective proteins in Lonomia obliqua caterpillar hemolymph and shows their protective effect on rat hippocampal neurons against apoptosis.

SARS-CoV-2 immune complex triggers human monocyte necroptosis

This study shows SARS-CoV-2 and its immune complexes induce monocyte necroptosis via RIPK3 and MLKL, with Syk kinase involvement, and links elevated LDH to COVID-19 pathogenesis.

OM-85 reduces SARS-COV-2 viral RNA expression in nasopharyngeal cells from COVID-19 patients

The paper shows OM-85 reduces SARS-CoV-2 N1 gene expression and increases RIG-I gene expression in nasopharyngeal cells from COVID-19 patients.

Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

This paper develops tau biosensors using Nanoluciferase technology to monitor tau conformational changes and seeding activity in living cells, identifying potential therapeutic targets for tauopathies.

A high throughput SEC23 functional interaction screen reveals a role for focal adhesion and extracellular matrix signalling in the regulation of COPII subunit SEC23A

The paper screens 378 cytoskeleton-associated proteins for functional interactions with COPII subunits SEC23A and SEC23B, identifying regulators linked to focal adhesion and ECM signaling.

Fluorescence-based 3D targeting of FIB-SEM acquisition of small volumes in large samples

The paper presents a method to precisely target FIB-SEM acquisition of fluorescently labeled cells in large samples using fluorescence preservation and confocal imaging.

Evaluating aroA gene essentiality and EPSP synthase vulnerability in Mycobacterium smegmatis under different nutritional conditions

This study evaluates the essentiality of the aroA gene and vulnerability of its enzyme EPSPS in Mycobacterium smegmatis under different nutritional conditions using gene knockout, knockdown, and kinetic assays.

Whole-body integration of gene expression and single-cell morphology

The paper integrates gene expression and single-cell morphology in Platynereis dumerilii to identify spatially coherent cell groups defined by transcription factors.

Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism

The paper demonstrates that endocytosis and late endosome trafficking regulate membrane allocation in Drosophila tracheal terminal cells during tubulogenesis.

Rupture of nuclear envelope in starfish oocytes proceeds by F-actin-driven segregation of pore-dense and pore-free membranes

This study shows that F-actin, driven by Arp2/3, causes nuclear envelope rupture in starfish oocytes by segregating pore-dense and pore-free membranes.

Studying the fate of tumor extracellular vesicles at high spatio-temporal resolution using the zebrafish embryo

This study tracks tumor extracellular vesicles in zebrafish embryos using fluorescent probes, showing their uptake by endothelial cells and macrophages and their behavior in vivo.