Benjamin K. Kesler

TrueSpot: A robust automated tool for quantifying signal puncta in fluorescent imaging

TrueSpot is an automated tool for quantifying signal puncta in fluorescent imaging, outperforming 2D methods in 3D spot detection.

TrueProbes: Quantitative Single-Molecule RNA-FISH Probe Design Improves RNA Detection

TrueProbes improves RNA detection by designing high-specificity smRNA-FISH probes using BLAST analysis and thermodynamic modeling, outperforming existing tools in computational and experimental metrics.

Transcriptional stochasticity reveals multiple mechanisms of long noncoding RNA regulation at the Xist - Tsix locus

The paper investigates how transcriptional stochasticity influences the regulation of Xist and Tsix at the XCI locus through different mechanisms based on nascent transcription levels.

A rate threshold mechanism regulates MAPK stress signaling and survival

The paper shows that stimulation rate, along with concentration, determines MAPK signaling and cell survival in budding yeast through a rate threshold mechanism.

Automated cell boundary and 3D nuclear segmentation of cells in suspension

The paper introduces CellDissect, a method for automated cell and nuclear segmentation in suspension cells using widefield images, and evaluates its accuracy against manual segmentation.