Enhancing and stabilizing a high-yield industrial Rhodobacter sphaeroides strain for coenzyme Q10 production
Mindong Liang, Xinwei He, Dongyuan Lv, Jing Liu, Kefeng Wang, Yingrui Hou, Weishan Wang, Linquan Bai, Guang Liu, Zhichun Zhu, Dan Li, Biqin Chen, Lixin Zhang, Gao‐Yi Tan
This paper enhances CoQ10 production in Rhodobacter sphaeroides by engineering the PrrAB system and stabilizing the strain through CRISPRi, achieving 3.6 g/L in pilot-scale fermentation.
bioRxiv · Jul 16, 2025 · PreprintMicrobiology