Gao‐Yi Tan

This paper develops a two-stage fermentation system using Clostridium pasteurianum and Rhodobacter sphaeroides to enhance hydrogen production while reducing inhibitory metabolite accumulation.

This paper enhances CoQ10 production in Rhodobacter sphaeroides by engineering the PrrAB system and stabilizing the strain through CRISPRi, achieving 3.6 g/L in pilot-scale fermentation.

The paper develops a method to efficiently clone large GC-rich BGCs from Streptomyces genomes using a CRISPR/Cas12a-based system, demonstrating its effectiveness with a 87-kb BGC.