C. A. Marques

Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei

This study uses Nanopore sequencing to show that DNA replication compartmentalization in Trypanosoma brucei affects genome stability and instability by influencing VSG gene recombination and chromosome stability.

R-loops acted on by RNase H1 are a determinant of chromosome length-associated DNA replication timing and genome stability in Leishmania

This study shows that R-loops and RNase H1 regulate DNA replication timing and genome stability in Leishmania, linking R-loop homeostasis to chromosome size and replication order.

Immunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei reveals conserved and novel activities, including in host immune evasion by antigenic variation

The paper identifies 602 RNA-DNA hybrid interactors in Trypanosoma brucei using immunoprecipitation and mass spectrometry, revealing conserved and novel functions in DNA repair and immune evasion.

RAD51-mediated R-loop formation acts to repair transcription-associated DNA breaks driving antigenic variation in Trypanosoma brucei

This study shows RAD51 interacts with RNA-DNA hybrids and repairs DNA breaks at the transcribed VSG in T. brucei, linking RNA-DNA hybrids to antigenic variation.

Profiling the bloodstream form and procyclic form Trypanosoma brucei cell cycle using single cell transcriptomics

This study uses single-cell transcriptomics to profile cell cycle dynamics in two forms of Trypanosoma brucei, identifying conserved and form-specific gene expression patterns.