L. Lemgruber

Immunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei reveals conserved and novel activities, including in host immune evasion by antigenic variation

The paper identifies 602 RNA-DNA hybrid interactors in Trypanosoma brucei using immunoprecipitation and mass spectrometry, revealing conserved and novel functions in DNA repair and immune evasion.

RAD51-mediated R-loop formation acts to repair transcription-associated DNA breaks driving antigenic variation in Trypanosoma brucei

This study shows RAD51 interacts with RNA-DNA hybrids and repairs DNA breaks at the transcribed VSG in T. brucei, linking RNA-DNA hybrids to antigenic variation.

Evolution of lineage-specific trafficking proteins and a novel post-Golgi trafficking pathway in Apicomplexa

This study identifies lineage-specific membrane-trafficking paralogs in Apicomplexa, revealing a novel post-Golgi trafficking pathway involved in parasite invasion.

Widespread roles of Trypanosoma brucei ATR in nuclear genome function and transmission are linked to R-loops

This study shows that ATR in Trypanosoma brucei is involved in genome maintenance, R-loop regulation, and chromosome segregation, despite DNA damage and continued cell division after ATR depletion.

Depletion of voltage-dependent anion channel (VDAC) of Toxoplasma gondii affects multiple mitochondrial functions, but not calcium signalling.

This study shows that VDAC in Toxoplasma gondii is essential for mitochondrial protein import and metabolite transfer but does not regulate calcium signaling.

Genome maintenance functions of Trypanosoma brucei DNA Polymerase N include telomere association and a role in antigenic variation

This study identifies a dual role for DNA Polymerase N in Trypanosoma brucei, showing its involvement in telomere maintenance and antigenic variation, contributing to genome stability and immune evasion.

Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion

This study shows that F-actin is essential for nuclear passage during host cell invasion by apicomplexan parasites, stabilizing the junction and facilitating nuclear movement.

Trypanosoma brucei ribonuclease H2A is an essential enzyme that resolves R-loops associated with transcription initiation and antigenic variation

This study shows that TbRH2A is essential for resolving R-loops and preventing DNA damage in T. brucei, with distinct roles in RNA Pol II and Pol I transcription.

RibonucleaseH1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion

This study shows RNase H1 regulates R-loop formation in Trypanosoma brucei VSG expression sites, linking R-loops to immune evasion through increased VSG coat switching and genome damage.