A strategy to address dissociation-induced compositional and transcriptional bias for single-cell analysis of the human mammary gland
L. K. Engelbrecht, A.-J. Twigger, H. M. Ganz, C. J. Gabka, A. R. Bausch, H. Lickert, M. Sterr, I. Kunze, W. T. Khaled, C. H. Scheel
The paper presents a strategy to minimize dissociation-induced biases in single-cell analysis of human mammary glands by comparing different dissociation methods using single-cell RNA sequencing and flow cytometry.
bioRxiv · Feb 11, 2021 · PreprintCell Biology