Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells
V. Glaser, C. L. Flugel, J. Kath, W. Du, V. Drosdek, C. Franke, M. Stein, A. Pruss, M. Schmueck-Henneresse, H.-D. Volk, P. Reinke, D. L. Wagner
This paper combines CRISPR nucleases to simultaneously knock-in and base edit CAR T cells, reducing translocations to 1.5% and improving safety.
bioRxiv · Nov 11, 2022Bioengineering