M. Schmueck-Henneresse

This paper develops a method to integrate ζ-deficient CARs into the CD3ζ gene to create functional CAR-T and CAR-NK cells with improved safety and efficacy for off-the-shelf therapies.

This study shows that matching or engineering HLA-I and HLA-II improves the efficacy of allogeneic regulatory T cells for immunoregulation.

This paper combines CRISPR nucleases to simultaneously knock-in and base edit CAR T cells, reducing translocations to 1.5% and improving safety.

This paper presents a virus-free method to generate CAR T cells by inserting CARs into the TRAC locus using CRISPR-Cas and HDR, achieving up to 68% TCR-to-CAR replacement.

The paper identifies a widespread memory/effector T cell response and regulatory T cell presence against SpCas9 in healthy humans, with implications for gene therapy.