Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells
D. C. Stephens, A. Crabtree, H. K. Beasley, E. Garza-Lopez, K. Neikirk, M. Mungai, L. Vang, Z. Vue, N. Vue, A. G. Marshall, K. Turner, J.-q. Shao, S. A. Murray, J. A. Gaddy, C. Wanjalla, , A. Hinton
This paper describes a protocol for using proximity ligation assays (PLA) to study protein-protein interactions in mitochondria-endoplasmic reticulum contact sites (MERCs) in mouse skeletal muscle tissue and cells.
bioRxiv · May 20, 2023Cell Biology